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KMID : 0380220070400020286
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Journal of Biochemistry and Molecular Biology
2007 Volume.40 No. 2 p.286 ~ p.289
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Suppression Subtractive Hybridization Identifies Novel Transcripts in Regenerating Hydra littoralis
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Stout Thomas
McFarland Trevor
Appukuttan Binoy
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Abstract
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Despite considerable interest in the biologic processes of regeneration and stem cell activation, little is known about the genes involved in these transformative events. In a Hydra littoralis model of regeneration, we employed a rapid shotgun suppression subtractive hybridization strategy to identify genes that are uniquely expressed in regenerating tissue. With an adaptor-PCR based technique, 16 candidate transcripts were identified, 15 were confirmed unique to mRNA isolated from hydra undergoing regeneration. Of these, 6 were undescribed in GenBank and allied expressed sequence tag (EST) databases (GenBank + EMBL + DDBJ + PDB and the Hydra EST database). BLAST analysis of these sequences identified remarkably similar sequences in anonymous ESTs found in a wide variety of animal species.
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KEYWORD
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Differential transcription, Gene, Hydra, Tissue regeneration
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